Data pro 2018/2019
Biochemistry: Laboratory
Kredity | 4 |
Rozsah | 0 / 0 / 6 |
Examinace | KZ |
Jazyk výuky | angličtina |
Úroveň | bakalářský předmět |
Garant |
doc. Dr. Ing. Zuzana Novotná RNDr. Jarmila Zídková, CSc. |
Anotace
None
Sylabus
1. Identification of amino acids by paper electrophoresis
2. Identification of proteins by SDS PAGE (polyacrylamide gel electrophoresis)
3. Determination of tripeptide sequence
4. Isolation of DNA from spleen
5. Spectrophotometric determination of protein and DNA concentrations in a mixed sample
6. Separation of macromolecules by gel filtration chromatography
7. Immunoprecipitation reactions in solution
8. Separation of trypsin by ion-exchange chromatography
9. Influence of reaction conditions on trypsin activity
10. Determination of the Michaelis constant using chromogenic substrate
11. Immobilisation of yeasts to alginate gel
12. Titration curves of amino acids
2. Identification of proteins by SDS PAGE (polyacrylamide gel electrophoresis)
3. Determination of tripeptide sequence
4. Isolation of DNA from spleen
5. Spectrophotometric determination of protein and DNA concentrations in a mixed sample
6. Separation of macromolecules by gel filtration chromatography
7. Immunoprecipitation reactions in solution
8. Separation of trypsin by ion-exchange chromatography
9. Influence of reaction conditions on trypsin activity
10. Determination of the Michaelis constant using chromogenic substrate
11. Immobilisation of yeasts to alginate gel
12. Titration curves of amino acids
Literatura
None